Journal: Communications Biology
Article Title: Ursodeoxycholic acid alleviates aortic aneurysm and dissection through the intestinal farnesoid X receptor/ceramide synthase 2 axis
doi: 10.1038/s42003-025-08403-2
Figure Lengend Snippet: A Differential lipid heatmap of ileal tissue. ( n = 6 per group). B Kyoto Encyclopedia of Genes and Genomes (KEGG) Bubble Chart for inter group differential metabolites ( n = 6 per group). C Quantification of serum ceramides in normal mice, AAD mice and UDCA mice ( n = 8 per group). D Pearson’s correlation analysis between serum FGF15 and C20 ceramide levels in mice. E – G Detection results of ceramide synthase expression in the AAD model induced by BAPN. ( n = 6, per group). H – J Detection results of ceramide synthase expression in the AAD model induced by AngII. (n = 6, per group) ( K – L ) Western blot results confirm that treatment with the FXR agonist GW4064 (10 μM) or DMSO enhances the expression of Cers2 in primary enterocytes ( n = 4 per group). M Motif analysis shows FXR putatively bound to Cers2 motif. N Chip-qPCR was performed in mice treated with GW4064 (80 mg/kg/d) or DMSO ( n = 3 per group). O Results of the dual-luciferase assay ( n = 3 per group). Data in L and N are shown as the mean ± SEM. Statistical analysis was performed using two-tailed unpaired Student’s t test. Data in ( C , E – J , O ) are shown as the mean ± SEM. Statistical analysis was performed using a one-way anova test with Dunnett’s multiple comparisons posttest. (* p < 0.05, ** p < 0.01, *** p < 0.001).
Article Snippet: Establishment of the AAD mouse model using a combination of β-aminopropionitrile (BAPN) (HY-Y1750, MCE, Shanghai, China) and Angiotensin II AngII (HY-13948, MCE, Shanghai, China).
Techniques: Expressing, Western Blot, ChIP-qPCR, Luciferase, Two Tailed Test
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